Regulatory Landscaping: How Enhancer-Promoter Communication Is Sculpted in 3D

During embryogenesis, precise gene transcription in space and time requires that distal enhancers and promoters communicate by physical proximity within gene regulatory landscapes. To achieve this, regulatory landscapes fold in nuclear space, creating complex 3D structures that influence enhancer-promoter communication and gene expression and that, when disrupted, can cause disease.

enhancers and promoters construct regulatory landscapes

multiple scales of 3D chromatin structure sculpt their communication

these same forces spatially separate regulatory landscapes by functional state, thereby creating higher-order compartments that reconfigure during development to enable proper enhancer-promoter communication