Pioneering, chromatin remodeling, and epigenetic constraint in early T-cell gene regulation by SPI1 (PU.1)
Genome Res. 2018. Published by Cold Spring Harbor Laboratory Press
effective pioneering at closed chromatin sites also depends on requirements beyond site recognition served by non-DNA binding domains of PU.1.
PU.1 is known to act as a pioneer in the role of a lineage determining factor that initiates and sustains identity of myeloid cells
Criteria for pioneering involve the ability to open closed chromatin and to initiate occupancy of a cis-regulatory site by a complex of transcription factors
Common Design 